Human Nuclear Antigen Antibody

Biorbyt

Human Nuclear Antigen Antibody

16

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nuclear Antigen antibody to be titered up or down for optimal performance.. Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunocytochemistry (acetone-fixed cells): 1-2ug/ml for 30 min at RT,Immunohistochemistry (Frozen): 1-2ug/ml for 30 min at RT FACS, ICC, IF, IHC-Fr, IP

Research Use Only

Monoclonal

235-1

Unconjugated

Mouse

IgG1

This human nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Human

-20°C,2°C to 8°C

12352203