Human OLIG2(Oligodendrocyte Lineage Transcription Factor 2) ELISA Kit

Biorbyt

Human OLIG2(Oligodendrocyte Lineage Transcription Factor 2) ELISA Kit

10

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OLIG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OLIG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OLIG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human OLIG2 in the samples is then determined by comparing the OD of the samples to the standard curve

15.63-1000 pg/mL

5.5 pg/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oligodendrocyte Lineage Transcription Factor 2(OLIG2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Oligodendrocyte Lineage Transcription Factor 2(OLIG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oligodendrocyte Lineage Transcription Factor 2(OLIG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Oligodendrocyte Lineage Transcription Factor 2(OLIG2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Human

41116158