Human PH20, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human PH20, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human PH20 / SPAM1 Protein, His Tag (active enzyme) DMF Filed
PH0-H5225
Product group Molecular Biology
Overview
- SupplierACRObiosystems
- Product NameHuman PH20 / SPAM1 Protein, His Tag (active enzyme) DMF Filed
- Delivery Days Customer4
- Category SupplierEnzymes
- CertificationResearch Use Only
- Scientific DescriptionCharacteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Description: Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
- Shelf life instruction● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
- Storage Instruction-20℃
- UNSPSC41116133