Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit

Biorbyt

Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit

10

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve

78.13-5000 pg/mL

29 pg/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphorylated Neurofilament H(pNF-H). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphorylated Neurofilament H(pNF-H). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphorylated Neurofilament H(pNF-H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphorylated Neurofilament H(pNF-H) in the samples is then determined by comparing the OD of the samples to the standard curve.

41116158