Deglycosylation of GTX65641-pro Progranulin (human) protein. To examine the deglycosylation of human Progranulin, 1 μg of human progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37oC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (human) antibody.
Deglycosylation of GTX65641-pro Progranulin (human) protein. To examine the deglycosylation of human Progranulin, 1 μg of human progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37oC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (human) antibody.
Human Progranulins protein (active)
GTX65641-PRO
ApplicationsFunctional Assay
Product group Proteins / Signaling Molecules
Overview
- SupplierGeneTex
- Product NameHuman Progranulins protein (active)
- Delivery Days Customer9
- Application Supplier NoteActivates phospho-ERK1/2 in neuronal mouse P19 cells. Regulates food intake and body weight.
- ApplicationsFunctional Assay
- CertificationResearch Use Only
- ConjugateUnconjugated
- Gene ID2896
- Target nameGRN
- Target descriptiongranulin precursor
- Target synonymsCLN11, FTD2, GEP, GP88, PCDGF, PEPI, PGRN, progranulin, PC cell-derived growth factor, acrogranin, epithelin, glycoprotein 88, glycoprotein of 88 Kda, granulin-epithelin, granulins, proepithelin
- Storage Instruction-20°C
- UNSPSC41116120
- SpeciesHuman