The figure shows a typical standard curve for the soluble SEMA4D ELISA. The immunoassay is calibrated against recombinant human Semaphorin 4D.
The figure shows a typical standard curve for the soluble SEMA4D ELISA. The immunoassay is calibrated against recombinant human Semaphorin 4D.
Human soluble Semaphorin 4D ELISA
BI-20405
Assay Sample TypeEDTA plasma, citrate plasma, heparın plasma, cell culture supernatant
Product group Assays
Overview
- SupplierBiomedica
- Product NameHuman soluble Semaphorin 4D ELISA
- Delivery Days Customer7
- ApplicationsELISA
- Assay Detection Range0 – 2 000 pmol/l (= 0 – 157.8 ng/ml)
- Assay PrecisionIn-between-run (n=11): ≤ 11 % CV. Within-run (n=5): ≤ 8 % CV
- Assay Sample TypeEDTA plasma, citrate plasma, heparın plasma, cell culture supernatant
- Assay Sensitivity12 pmol/l (= 0.94 ng/ml)
- Assay SpecificityEndogenous and recombinant human soluble Semaphorin 4D.
- Assay Test PrincipleThis Smephorin 4D ELISA kit is a sandwich enzyme immunoassay for the quantitative determination of soluble Semaphorin 4D in human plasma and cell culture supernatant.
- Assay Time3 h / 1 h / 30 min
- CertificationResearch Use Only
- Scientific DescriptionThe Semaphorin 4D ELISA kit is a 4.5 hour, 96-well sandwich ELISA for the quantitative determination of soluble Sempahorin 4D (SEMA4D) in EDTA plasma, citrate plasma, heparın plasma and cell culture supernatant. The assay employs human plasma-based standards to ensure the measurement of biologically reliable data. The soluble SEMA4D ELISA kit uses highly purified, epitope mapped antibodies. The antibodies utilized in the soluble Semaphorin-4D ELISA kit bind to AA30-AA34 and AA238-AA214 of soluble Semaphorin 4D.
- UNSPSC41116133
References
- Aberer F, Pferschy PN, Tripolt NJ, et al. Hypoglycaemia leads to a delayed increase in platelet and coagulation activation markers in people with type 2 diabetes treated with metformin only: Results from a stepwise hypoglycaemic clamp study. Diabetes Obes Metab. 2020;22(2):212-221.Read more