Human STIP1 protein, His tag (active)

GeneTex

Human STIP1 protein, His tag (active)

9

4 microM HOP Protein, when added to 2 microM AHA1 Protein (Aha1)-activated HSP90 (2 microM; His-tagged HSP90 beta) in 33 mM HEPES (pH 7.2) with 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 microl reaction at 37 C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic HSP90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).

Functional Assay

Research Use Only

Unconjugated

Protein S100-A11

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009]

-20°C

12352202