
WB analysis of GTX00278-pro Human TFPI2 protein (active).
Human TFPI2 protein, His tag (active)
GTX00278-PRO
ApplicationsFunctional Assay
Product group Proteins / Signaling Molecules
Protein IDP48307
Overview
- SupplierGeneTex
- Product NameHuman TFPI2 protein (active)
- Delivery Days Customer9
- Application Supplier NoteTissue Factor Pathway Inhibitor 2 (TFPI2) takes part in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. TFPI2 does not have any influence on thrombin. TFPI2 also can inhibit MMP activity, which can hydrolyze gelatin under certain conditions. Thus, the activity of TFPI2 can be measured by inhibit MMP-2 hydrolyze gelatin. Gelatin zymography is mainly used for the detection of the gelatinases, 2microg/ml was denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate-polyacrylamide gel (SDS-PAGE; 8% gels) containing gelatin (1 mg/ml) with nonreducing conditions. After renaturation, incubate with various concentrations of recombinant human TFPI2, then staining with coomassie brilliant blue G250, active MMP-2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel; if the activity of MMP-2 inhibit by TFPI2, there was none white binds on the gel. MMP-2 can be inhibited by recombinant human TFPI2 at least 5microg/ml.
- ApplicationsFunctional Assay
- CertificationResearch Use Only
- ConjugateUnconjugated
- Protein IDP48307
- Protein NameTissue factor pathway inhibitor 2
- Scientific DescriptionThis gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352202