Human UGCG protein, His tag

GeneTex

Human UGCG protein (active)

9

UDP Glucose Ceramide Glucosyltransferase (UGCG) is an enzyme which catalyzes the first glycosylation step in glycosphingolipid biosynthesis. It belongs to the glycosyltransferase 2 family. UGCG is widely expressed and transcription is upregulated during keratinocyte differentiation. Besides, Large Multifunctional Peptidase 2 (LMP2) has been identified as an interactor of UGCG, thus a binding ELISA assay was conducted to detect the interaction of recombinant human UGCG and recombinant human LMP2. Briefly, UGCG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 microl were then transferred to LMP2-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-UGCG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 microl stop solution to the wells and read at 450nm immediately. The binding activity of UGCG and LMP2 was in a dose dependent manner.

Functional Assay

Research Use Only

Unconjugated

Ceramide glucosyltransferase

This gene encodes an enzyme that catalyzes the first glycosylation step in the biosynthesis of glycosphingolipids, which are membrane components containing lipid and sugar moieties. The product of this reaction is glucosylceramide, which is the core structure of many glycosphingolipids. [provided by RefSeq, Dec 2014]

-20°C or -80°C,2°C to 8°C

12352202