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Lane 1: A431 Cells; Probed with Huntingtin (1F10) Monoclonal Antibody (bsm-54305R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: A431 Cells; Probed with Huntingtin (1F10) Monoclonal Antibody (bsm-54305R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: A431 Cells; Probed with Huntingtin (1F10) Monoclonal Antibody (bsm-54305R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Huntingtin Recombinant Antibody

BSM-54305R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetHTT
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Huntingtin (1F10) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID3064
  • Target name
    HTT
  • Target description
    huntingtin
  • Target synonyms
    HD; huntingtin; huntington disease protein; IT15; LOMARS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42858
  • Protein Name
    Huntingtin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of Huntingtin/HTT using anti-Huntingtin/HTT antibody (A00134-2). Huntingtin/HTT was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Huntingtin/HTT Antibody (A00134-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Immunohistochemistry analysis in human cerebral cortex and liver tissues using Anti-HTT antibody. Corresponding HTT RNA-seq data are presented for the same tissues.
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