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HUPF2 / UPF2 Antibody

LS-C748461
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetUPF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HUPF2 / UPF2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 66kDa/147kDa, while the observed MW by Western blot was 180kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 66kDa/147kDa, while the observed MW by Western blot was 180kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26019
  • Target name
    UPF2
  • Target description
    UPF2 regulator of nonsense mediated mRNA decay
  • Target synonyms
    HUPF2, RENT2, smg-3, regulator of nonsense transcripts 2, FRS2/UPF2/LEMD3 fusion, LEMD3/UPF2 fusion, UPF2 regulator of nonsense transcripts homolog, nonsense mRNA reducing factor 2, smg-3 homolog, nonsense mediated mRNA decay factor, up-frameshift suppressor 2 homolog, yeast Upf2p homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HUPF2/RENT2/UPF2 using anti-HUPF2/RENT2/UPF2 antibody (A03627-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HUPF2/RENT2/UPF2 antigen affinity purified polyclonal antibody (Catalog # A03627-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HUPF2/RENT2/UPF2 at approximately 170 kDa. The expected band size for HUPF2/RENT2/UPF2 is at 170 kDa.
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