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WB analysis of various samples using GTX12396 HYAL1 antibody. Lane 1 : NRK cell lysate Lane 2 : mouse liver tissue lysate Lane 3 : mouse kidney tissue lysate Lane 4 : SP2/0 cell lysate
WB analysis of various samples using GTX12396 HYAL1 antibody. Lane 1 : NRK cell lysate Lane 2 : mouse liver tissue lysate Lane 3 : mouse kidney tissue lysate Lane 4 : SP2/0 cell lysate
WB analysis of various samples using GTX12396 HYAL1 antibody. Lane 1 : NRK cell lysate Lane 2 : mouse liver tissue lysate Lane 3 : mouse kidney tissue lysate Lane 4 : SP2/0 cell lysate

HYAL1 antibody

GTX12396
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse, Rat
TargetHyal1
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Overview

  • Supplier
    GeneTex
  • Product Name
    HYAL1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID15586
  • Target name
    Hyal1
  • Target description
    hyaluronoglucosaminidase 1
  • Target synonyms
    Fus2, Hya1, Hyal-1, Nat6, hyaluronidase-1, N-acetyltransferase 6, Protein fus-2, Protein fusion-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ91ZJ9
  • Protein Name
    Hyaluronidase-1
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HYAL1 using anti-HYAL1 antibody (A04099-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: rat RH-35 whole cell lysates, Lane 3: mouse HEPA1-6 whole cell lysates, Lane 4: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HYAL1 antigen affinity purified polyclonal antibody (Catalog # A04099-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HYAL1 at approximately 60KD. The expected band size for HYAL1 is at 60KD.
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TargetHyal1
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