IHC-P analysis of human gastric carcinoma tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of human stomach tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22866/GTX22866_1182_IHC-P_w_23060620_319.webp "IHC-P analysis of human stomach tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![ICC/IF analysis of A2058 Cells using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22866/GTX22866_528_ICC-IF_w_23060620_819.webp "ICC/IF analysis of A2058 Cells using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
IHC-P analysis of human gastric carcinoma tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
Hydrogen Potassium ATPase Beta antibody [2G11]
GTX22866
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Guinea Pig, Human, Mammals, Mouse, Porcine, Rabbit, Rodent, Rat
TargetATP4B
Overview
- SupplierGeneTex
- Product NameHydrogen Potassium ATPase Beta antibody [2G11]
- Delivery Days Customer9
- Antibody SpecificityThis antibody has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site. And it recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cyto
- Application Supplier NoteWB: 1:4,000. ICC/IF: 1:2,000. IHC-P: 1:2000. IHC-Fr: 1:2000. FACS: 1/50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2G11
- ConjugateUnconjugated
- Gene ID496
- Target nameATP4B
- Target descriptionATPase H+/K+ transporting subunit beta
- Target synonymsATP6B; ATPase H+/K+ transporting beta subunit; ATPase, H+/K+ exchanging, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide; gastric H(+)/K(+) ATPase subunit beta; gastric H+/K+ ATPase beta subunit; gastric hydrogen-potassium ATPase, beta; potassium-transporting ATPase beta chain; potassium-transporting ATPase subunit beta; proton pump beta chain
- HostMouse
- IsotypeIgG1
- Protein IDP51164
- Protein NamePotassium-transporting ATPase subunit beta
- Scientific DescriptionThe protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]
- ReactivityBovine, Canine, Guinea Pig, Human, Mammals, Mouse, Porcine, Rabbit, Rodent, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- High Hepcidin Levels Promote Abnormal Iron Metabolism and Ferroptosis in Chronic Atrophic Gastritis.Read more