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HYOU1 / ORP150 Antibody

LS-C747440
LifeSpan BioSciences
ApplicationsImmunoFluorescence
Product group Antibodies
TargetHYOU1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HYOU1 / ORP150 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 62kDa/111kDa.
  • Applications
    ImmunoFluorescence
  • Applications Supplier
    IF (1:50 - 1:200) The predicted MW is 62kDa/111kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10525
  • Target name
    HYOU1
  • Target description
    hypoxia up-regulated 1
  • Target synonyms
    150 kDa oxygen-regulated protein; 170 kDa glucose-regulated protein; epididymis secretory sperm binding protein; Grp170; GRP-170; HSP12A; hypoxia up-regulated protein 1; IMD59; ORP150; ORP-150; oxygen regulated protein (150kD)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ORP150/HYOU1 using anti-ORP150/HYOU1 antibody (A04934-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORP150/HYOU1 antigen affinity purified polyclonal antibody (Catalog # A04934-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORP150/HYOU1 at approximately 150 kDa. The expected band size for ORP150/HYOU1 is at 150 kDa.
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