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ProductsBack/I-FABP / FABP2 Antibody

I-FABP / FABP2 Antibody

LS-C23504
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFABP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    I-FABP / FABP2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in staining cells containing I-FABP in cells cultured on cover slip (Immunohistochemistry), Western Blot and ELISA. Immunohistochemistry: 1:10. Western Blot: 1:10.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:10), WB (1:10) Suitable for use in staining cells containing I-FABP in cells cultured on cover slip (Immunohistochemistry), Western Blot and ELISA. Immunohistochemistry: 1:10. Western Blot: 1:10.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2169
  • Target name
    FABP2
  • Target description
    fatty acid binding protein 2
  • Target synonyms
    FABPI; fatty acid binding protein 2, intestinal; fatty acid-binding protein, intestinal; I-FABP; intestinal-type fatty acid-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FABP2/I-FABP using anti-FABP2/I-FABP antibody (PB9943). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP2/I-FABP antigen affinity purified polyclonal antibody (Catalog # PB9943) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP2/I-FABP at approximately 15 kDa. The expected band size for FABP2/I-FABP is at 15 kDa.
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