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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA133408(FABP2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA133408(FABP2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA133408(FABP2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)

FABP2 Antibody

CSB-PA133408
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFABP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    FABP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2169
  • Target name
    FABP2
  • Target description
    fatty acid binding protein 2
  • Target synonyms
    FABPI; fatty acid binding protein 2, intestinal; fatty acid-binding protein, intestinal; I-FABP; intestinal-type fatty acid-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12104
  • Protein Name
    Fatty acid-binding protein, intestinal
  • Scientific Description
    The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FABP2/I-FABP using anti-FABP2/I-FABP antibody (PB9943). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP2/I-FABP antigen affinity purified polyclonal antibody (Catalog # PB9943) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP2/I-FABP at approximately 15 kDa. The expected band size for FABP2/I-FABP is at 15 kDa.
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