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ICC/IF analysis of HepG2 cells using GTX87187 IARS2 antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of HepG2 cells using GTX87187 IARS2 antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of HepG2 cells using GTX87187 IARS2 antibody. The picture on the right is blocked with the synthesized peptide.

IARS2 antibody

GTX87187
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIARS2
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Overview

  • Supplier
    GeneTex
  • Product Name
    IARS2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. ICC/IF: 1:100~1:500. IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55699
  • Target name
    IARS2
  • Target description
    isoleucyl-tRNA synthetase 2, mitochondrial
  • Target synonyms
    CAGSSS; ILERS; isoleucine tRNA ligase 2, mitochondrial; isoleucine--tRNA ligase, mitochondrial; isoleucine-tRNA synthetase 2, mitochondrial; isoleucyl-tRNA synthetase, mitochondrial; mitochondrial isoleucine tRNA synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NSE4
  • Protein Name
    Isoleucine--tRNA ligase, mitochondrial
  • Scientific Description
    Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IARS2 using anti-IARS2 antibody (A09580-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IARS2 antigen affinity purified polyclonal antibody (Catalog # A09580-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IARS2 at approximately 114 kDa. The expected band size for IARS2 is at 114 kDa.
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