More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI3F12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA. Positive lysates LY418008 (100ug) and LC418008 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA. Positive lysates LY418008 (100ug) and LC418008 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA. Positive lysates LY418008 (100ug) and LC418008 (20ug) can be purchased separately from OriGene.

ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI3F12]

CF500063
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Monkey
TargetDFFA
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI3F12]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3F12
  • Gene ID1676
  • Target name
    DFFA
  • Target description
    DNA fragmentation factor subunit alpha
  • Target synonyms
    DFF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, inhibitor of CAD
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO00273
  • Protein Name
    DNA fragmentation factor subunit alpha
  • Scientific Description
    Carrier-free (BSA/glycerol-free) DFF45 mouse monoclonal antibody, clone OTI3F12 (formerly 3F12)
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Bioss
DFFA Recombinant Antibody, AbBy Fluor-555 ConjugatedBSM-62067R-BF555
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetDFFA
  • SizePrice
Product group Antibodies
Assay Genie
SLC28A3 AntibodyCAB10320
ReactivityHuman
TargetDFFA
  • SizePrice
Western Blot analysis of 3T3 cells using ICAD Polyclonal Antibody
Product group Antibodies
Cusabio
DFFA AntibodyCSB-PA002996
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDFFA
  • SizePrice
Product group Antibodies
Biomatik
DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal AntibodyCAU21479
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetDFFA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-DFFA Antibody144-12431
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetDFFA
  • SizePrice
Anti-DFFA Antibody
Product group Antibodies
Antibodies.com
Anti-DFFA AntibodyA96215
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DFFA / ICAD / DFF45 AntibodyLS-C766039
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetDFFA
  • SizePrice
Product group Antibodies
MedChem Express
ICAD Antibody (YA2262, Rabbit)HY-P82517
TargetDFFA
  • SizePrice
Immunohistochemical staining of human urinary bladder shows nuclear positivity in urothelial cells.
Product group Antibodies
Atlas Antibodies
Anti-DFFA AntibodyHPA018859
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetDFFA
  • SizePrice
Figure 1. Western blot analysis of DFFA/ICAD using anti-DFFA/ICAD antibody (A03671-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: rat stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DFFA/ICAD antigen affinity purified polyclonal antibody (Catalog # A03671-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DFFA/ICAD at approximately 45KD. The expected band size for DFFA/ICAD is at 45KD.
Product group Antibodies
Boster Bio
Anti-DFFA/ICAD Antibody Picoband(r)A03671-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Rat
TargetDFFA
  • SizePrice