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ProductsBack/ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI5C4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DFFA (Cat# RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA(Cat# TA500062). Positive lysates [LY418008] (100ug) and [LC418008] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DFFA (Cat# RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA(Cat# TA500062). Positive lysates [LY418008] (100ug) and [LC418008] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DFFA (Cat# RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA(Cat# TA500062). Positive lysates [LY418008] (100ug) and [LC418008] (20ug) can be purchased separately from OriGene.

ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI5C4]

CF500062
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetDFFA
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI5C4]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5C4
  • Gene ID1676
  • Target name
    DFFA
  • Target description
    DNA fragmentation factor subunit alpha
  • Target synonyms
    DFF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, inhibitor of CAD
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO00273
  • Protein Name
    DNA fragmentation factor subunit alpha
  • Scientific Description
    Carrier-free (BSA/glycerol-free) DFF45 mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of DFFA/ICAD using anti-DFFA/ICAD antibody (A03671-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: rat stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DFFA/ICAD antigen affinity purified polyclonal antibody (Catalog # A03671-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DFFA/ICAD at approximately 45KD. The expected band size for DFFA/ICAD is at 45KD.
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