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ICAM2 Antibody (YA3007, Rabbit)

HY-P83262
MedChem Express
TargetICAM2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ICAM2 Antibody (YA3007, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3384
  • Target name
    ICAM2
  • Target description
    intercellular adhesion molecule 2
  • Target synonyms
    CD102, intercellular adhesion molecule 2, ICAM-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ICAM2 Antibody (YA3007) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3007), targeting ICAM2, with a predicted molecular weight of 31 kDa (observed band size: 50-60 kDa). ICAM2 Antibody (YA3007) can be used for WB, IHC-P, IP experiment in human, mouse background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ICAM2 using anti-ICAM2 antibody (A03965). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate, Lane 2: rat thymus tissue lysate, Lane 3: rat lung tissue lysate, Lane 4: rat heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM2 antigen affinity purified polyclonal antibody (Catalog # A03965) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM2 at approximately 55KD. The expected band size for ICAM2 is at 31KD.
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