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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA084116(ICAM5 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA084116(ICAM5 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA084116(ICAM5 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

ICAM5 Antibody

CSB-PA084116
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetICAM5
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Overview

  • Supplier
    Cusabio
  • Product Name
    ICAM5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7087
  • Target name
    ICAM5
  • Target description
    intercellular adhesion molecule 5
  • Target synonyms
    TLCN, TLN, intercellular adhesion molecule 5, ICAM-5, intercellular adhesion molecule 5, telencephalin, telencephalin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UMF0
  • Protein Name
    Intercellular adhesion molecule 5
  • Scientific Description
    The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ICAM5 using anti-ICAM5 antibody (A07187-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM5 antigen affinity purified polyclonal antibody (Catalog # A07187-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM5 at approximately 140 kDa. The expected band size for ICAM5 is at 97 kDa.
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