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ProductsBack/ID2 Polyclonal Antibody
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GIG8/ID2 Polyclonal Antibody, Unconjugated (bs-3515R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GIG8/ID2 Polyclonal Antibody, Unconjugated (bs-3515R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GIG8/ID2 Polyclonal Antibody, Unconjugated (bs-3515R) at 1:100 followed by conjugation to the secondary antibody and DAB staining

ID2 Polyclonal Antibody

BS-3515R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetID2
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Overview

  • Supplier
    Bioss
  • Product Name
    ID2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3398
  • Target name
    ID2
  • Target description
    inhibitor of DNA binding 2
  • Target synonyms
    GIG8, ID2A, ID2H, bHLHb26, DNA-binding protein inhibitor ID-2, DNA-binding protein inhibitor ID2, cell growth-inhibiting gene 8, class B basic helix-loop-helix protein 26, helix-loop-helix protein ID2, inhibitor of DNA binding 2, HLH protein, inhibitor of DNA binding 2, dominant negative helix-loop-helix protein, inhibitor of differentiation 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02363
  • Protein Name
    DNA-binding protein inhibitor ID-2
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of ID2 using anti-ID2 antibody (A00417-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ID2 antigen affinity purified polyclonal antibody (Catalog # A00417-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ID2 at approximately 15KD. The expected band size for ID2 is at 15KD.
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