![IHC-P analysis of human liver tissue using GTX84325 ID3 antibody [10D3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human liver tissue using GTX84325 ID3 antibody [10D3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84325/GTX84325_2612_IHC-P_w_23061420_100.webp)
IHC-P analysis of human liver tissue using GTX84325 ID3 antibody [10D3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
ID3 antibody [10D3]
GTX84325
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetID3
Overview
- SupplierGeneTex
- Product NameID3 antibody [10D3] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID10D3
- Concentration0.61 mg/ml
- ConjugateUnconjugated
- Gene ID3399
- Target nameID3
- Target descriptioninhibitor of DNA binding 3
- Target synonymsHEIR-1, bHLHb25, DNA-binding protein inhibitor ID-3, ID-like protein inhibitor HLH 1R21, class B basic helix-loop-helix protein 25, helix-loop-helix protein HEIR-1, inhibitor of DNA binding 3, HLH protein, inhibitor of DNA binding 3, dominant negative helix-loop-helix protein, inhibitor of differentiation 3
- HostMouse
- IsotypeIgG1
- Protein IDQ02535
- Protein NameDNA-binding protein inhibitor ID-3
- Scientific DescriptionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![WB analysis of various cell lines using GTX84325 ID3 antibody [10D3]. Loading : 10 ug per lane Dilution : 1:200 WB analysis of various cell lines using GTX84325 ID3 antibody [10D3]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84325/GTX84325_3520_WB_w_23061420_590.webp)
![FACS analysis of HeLa cells using GTX84325 ID3 antibody [10D3]. Red : Primary antibody Blue : Negative control antibody FACS analysis of HeLa cells using GTX84325 ID3 antibody [10D3]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84325/GTX84325_361_FACS_w_23061420_274.webp)
![FACS analysis of Jurkat cells using GTX84325 ID3 antibody [10D3]. Red : Primary antibody Blue : Negative control antibody FACS analysis of Jurkat cells using GTX84325 ID3 antibody [10D3]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84325/GTX84325_362_FACS_w_23061420_964.webp)
![WB analysis of HEK293T cells transfected with ID3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84325 ID3 antibody [10D3]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with ID3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84325 ID3 antibody [10D3]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84325/GTX84325_4273_WB_w_23061420_889.webp)

![ICC/IF analysis of COS7 cells transiently transfected with ID3 plasmid using GTX84320 ID3 antibody [15F4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84320/GTX84320_1030_ICCIF_w_23061420_658.webp)
![ICC/IF analysis of COS7 cells transiently transfected with ID3 plasmid using GTX84327 ID3 antibody [7B6].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84327/GTX84327_1031_ICCIF_w_23061420_503.webp)


