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ProductsBack/Goat anti-Insulysin / Insulinase, Biotinylated Antibody

Goat anti-Insulysin / Insulinase, Biotinylated Antibody

ORB377877
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Goat anti-Insulysin / Insulinase, Biotinylated Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    WB: Approx 120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.2). Recommended concentration: 0.3-1microg/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA: 1:32000, WB: 0.3-1 μg/ml ELISA, IHC, WB
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody against IDE conjugated to Biotin.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IDE using anti-IDE antibody (M01358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified monoclonal antibody (M01358) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDE at approximately 118 kDa. The expected band size for IDE is at 118 kDa.
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