Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT286] (GTX633625) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT286] (GTX633625) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633625/GTX633625_42569_20170615_WB_M_R_w_23061202_738.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT286] (GTX633625) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![IDE antibody [GT286] detects IDE protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. IDE antibody [GT286] (GTX633625) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633625/GTX633625_42569_20160920_IHC-P_M_w_23061202_936.webp "IDE antibody [GT286] detects IDE protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. IDE antibody [GT286] (GTX633625) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Wild-type (WT) and IDE knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT286] (GTX633625) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633625/GTX633625_42569_20170309_WB_KO_watermark_w_23061202_663.webp "Wild-type (WT) and IDE knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT286] (GTX633625) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT286] (GTX633625) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
IDE antibody [GT286]
GTX633625
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIDE
Overview
- SupplierGeneTex
- Product NameIDE antibody [GT286]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT286
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3416
- Target nameIDE
- Target descriptioninsulin degrading enzyme
- Target synonymsINSULYSIN, insulin-degrading enzyme, Abeta-degrading protease, insulin protease, insulinase
- HostMouse
- IsotypeIgG1
- Protein IDP14735
- Protein NameInsulin-degrading enzyme
- Scientific DescriptionThis gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Li F, Yang J, Villar VAM, et al. Loss of renal SNX5 results in impaired IDE activity and insulin resistance in mice. Diabetologia. 2018,61(3):727-737. doi: 10.1007/s00125-017-4482-1Read this paper
- Rao KN, Li L, Anand M, et al. Ablation of retinal ciliopathy protein RPGR results in altered photoreceptor ciliary composition. Sci Rep. 2015,5:11137. doi: 10.1038/srep11137Read this paper
Datasheet
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