IDE antibody [N3C1], Internal detects Ide protein at the myotome on whole-mount zebrafish embryos by immunohistochemical analysis.? Sample: Paraformaldehyde-fixed zebrafish embryos. IDE antibody [N3C1], Internal (GTX111664) dilution: 1:200.
![IDE antibody [N3C1], Internal detects IDE protein at cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. IDE antibody [N3C1], Internal (GTX111664) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111664/GTX111664_40065_IHC_R_w_23060500_971.webp "IDE antibody [N3C1], Internal detects IDE protein at cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. IDE antibody [N3C1], Internal (GTX111664) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Various extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111664/GTX111664_44433_20210910_WB_w_23060500_248.webp "Various extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![IDE antibody [N3C1], Internal detects IDE protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IDE stained by IDE antibody [N3C1], Internal (GTX111664) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX111664/GTX111664_44433_20211008_ICC_IF_w_23060500_661.webp "IDE antibody [N3C1], Internal detects IDE protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IDE stained by IDE antibody [N3C1], Internal (GTX111664) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Wild-type (WT) and IDE Kip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111664/GTX111664_44433_20210917_WB_KO_watermark_w_23060500_677.webp "Wild-type (WT) and IDE Kip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111664/GTX111664_40065_20170615_WB_M_R_w_23060500_709.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
IDE antibody [N3C1], Internal detects Ide protein at the myotome on whole-mount zebrafish embryos by immunohistochemical analysis.? Sample: Paraformaldehyde-fixed zebrafish embryos. IDE antibody [N3C1], Internal (GTX111664) dilution: 1:200.
IDE antibody [N3C1], Internal
GTX111664
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetIDE
Overview
- SupplierGeneTex
- Product NameIDE antibody [N3C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.6 mg/ml
- ConjugateUnconjugated
- Gene ID3416
- Target nameIDE
- Target descriptioninsulin degrading enzyme
- Target synonymsINSULYSIN, insulin-degrading enzyme, Abeta-degrading protease, insulin protease, insulinase
- HostRabbit
- IsotypeIgG
- Protein IDP14735
- Protein NameInsulin-degrading enzyme
- Scientific DescriptionThis gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Hamilton KA, Wang Y, Raefsky SM, et al. Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus. PLoS One. 2018,13(5):e0196223. doi: 10.1371/journal.pone.0196223Read this paper
Datasheet
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