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IDH3A Antibody

LS-C749645
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetIDH3A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IDH3A Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 31kDa/40kDa, while the observed MW by Western blot was 40kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 31kDa/40kDa, while the observed MW by Western blot was 40kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3419
  • Target name
    IDH3A
  • Target description
    isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha
  • Target synonyms
    H-IDH alpha; isocitrate dehydrogenase (NAD(+)) 3 alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitrate dehydrogenase 3 (NAD(+)) alpha; isocitrate dehydrogenase 3 (NAD+) alpha; isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH; RP90
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ILDR1 using anti-ILDR1 antibody (A09544-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILDR1 antigen affinity purified polyclonal antibody (Catalog # A09544-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ILDR1 at approximately 37 kDa. The expected band size for ILDR1 is at 40 kDa.
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