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IDO1 antibody

GTX113753
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetIDO1
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Overview

  • Supplier
    GeneTex
  • Product Name
    IDO1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3620
  • Target name
    IDO1
  • Target description
    indoleamine 2,3-dioxygenase 1
  • Target synonyms
    IDO, IDO-1, INDO, indoleamine 2,3-dioxygenase 1, indolamine 2,3 dioxygenase, indole 2,3-dioxygenase, indoleamine-pyrrole 2,3-dioxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14902
  • Protein Name
    Indoleamine 2,3-dioxygenase 1
  • Scientific Description
    Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yokosawa T, Sato A, Wakasugi K. Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells. Genes (Basel). 2020,11(12). doi: 10.3390/genes11121423
    Read this paper
  • Sreevalsan S, Döring M, Paszkowski-Rogacz M, et al. MLLT6 maintains PD-L1 expression and mediates tumor immune resistance. EMBO Rep. 2020,21(12):e50155. doi: 10.15252/embr.202050155
    Read this paper
  • Gualdoni GS, Jacobo PV, Sobarzo CM, et al. Role of indoleamine 2,3-dioxygenase in testicular immune-privilege. Sci Rep. 2019,9(1):15919. doi: 10.1038/s41598-019-52192-8
    Read this paper
  • Wang LT, Chiou SS, Chai CY, et al. Intestine-Specific Homeobox Gene ISX Integrates IL6 Signaling, Tryptophan Catabolism, and Immune Suppression. Cancer Res. 2017,77(15):4065-4077. doi: 10.1158/0008-5472.CAN-17-0090
    Read this paper

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Figure 1. Western blot analysis of IDO1 using anti-IDO1 antibody (PB9603). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO1 antigen affinity purified polyclonal antibody (Catalog # PB9603) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO1 at approximately 45KD. The expected band size for IDO1 is at 45KD.
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