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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDO1 antibody (GTX113753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDO1 antibody (GTX113753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDO1 antibody (GTX113753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IDO1 antibody

GTX113753
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Rat
TargetIDO1
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Overview

  • Supplier
    GeneTex
  • Product Name
    IDO1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3620
  • Target name
    IDO1
  • Target description
    indoleamine 2,3-dioxygenase 1
  • Target synonyms
    IDO, IDO-1, INDO, indoleamine 2,3-dioxygenase 1, indolamine 2,3 dioxygenase, indole 2,3-dioxygenase, indoleamine-pyrrole 2,3-dioxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14902
  • Protein Name
    Indoleamine 2,3-dioxygenase 1
  • Scientific Description
    Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.[supplied by OMIM]
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yokosawa T, Sato A, Wakasugi K. Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells. Genes (Basel). 2020,11(12). doi: 10.3390/genes11121423
    Read this paper
  • Sreevalsan S, Döring M, Paszkowski-Rogacz M, et al. MLLT6 maintains PD-L1 expression and mediates tumor immune resistance. EMBO Rep. 2020,21(12):e50155. doi: 10.15252/embr.202050155
    Read this paper
  • Gualdoni GS, Jacobo PV, Sobarzo CM, et al. Role of indoleamine 2,3-dioxygenase in testicular immune-privilege. Sci Rep. 2019,9(1):15919. doi: 10.1038/s41598-019-52192-8
    Read this paper
  • Wang LT, Chiou SS, Chai CY, et al. Intestine-Specific Homeobox Gene ISX Integrates IL6 Signaling, Tryptophan Catabolism, and Immune Suppression. Cancer Res. 2017,77(15):4065-4077. doi: 10.1158/0008-5472.CAN-17-0090
    Read this paper

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