IDO1 Fluorogenic Inhibitor Screening Assay Kit

BPS Bioscience

IDO1 Fluorogenic Inhibitor Screening Assay Kit

7

Neutralisation/Blocking

Research Use Only

The IDO1 Fluorogenic Inhibitor Screening Assay Kit is designed to measure enzyme inhibition of indoleamine 2,3-dioxygenase 1 (IDO1). The IDO1 Fluorogenic Inhibitor Screening Assay Kit is simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hour incubation at room temperature, the fluorescence solution is added and incubated at 37°C for four hours. Activity is measured by reading sample fluorescence at lambda=510 nm following excitation of the reaction product at lambda=400 nm.

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