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Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate All lanes: IFI44L antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 19 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate All lanes: IFI44L antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 19 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate All lanes: IFI44L antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 19 kDa Observed band size: 52 kDa

IFI44L Antibody

CSB-PA011015LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetIFI44L
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Overview

  • Supplier
    Cusabio
  • Product Name
    IFI44L Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10964
  • Target name
    IFI44L
  • Target description
    interferon induced protein 44 like
  • Target synonyms
    C1orf29; GS3686; interferon-induced protein 44-like; TLDC5B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53G44
  • Protein Name
    Interferon-induced protein 44-like
  • Scientific Description
    Exhibits a low antiviral activity against hepatitis C virus.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IFI44L using anti-IFI44L antibody (A10975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: mouse NRK whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFI44L antigen affinity purified polyclonal antibody (A10975-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFI44L at approximately 51 kDa. The expected band size for IFI44L is at 51 kDa.
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