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ProductsBack/Goat anti-IFNAR1 (aa353-364) Antibody

Goat anti-IFNAR1 (aa353-364) Antibody

ORB107680
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIFNAR1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    IFNAR1 antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    WB: Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_000620.2). Recommended concentration: 0.3-1microg/ml.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA: 1:128000, WB: 0.3-1 μg/ml ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3454
  • Target name
    IFNAR1
  • Target description
    interferon alpha and beta receptor subunit 1
  • Target synonyms
    AVP, IFN-alpha-REC, IFNAR, IFNBR, IFRC, IMD106, interferon alpha/beta receptor 1, CRF2-1, IFN-R-1, IFN-alpha/beta receptor 1, IFNalpha/beta receptor 1, alpha-type antiviral protein, beta-type antiviral protein, cytokine receptor class-II member 1, cytokine receptor family 2 member 1, interferon (alpha, beta and omega) receptor 1, interferon receptor 1, interferon-alpha/beta receptor alpha chain, interferon-beta receptor 1, type I interferon receptor 1
  • Host
    Goat
  • Scientific Description
    Goat polyclonal to IFNAR1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IFNAR1 using anti-IFNAR1 antibody (A00306-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNAR1 antigen affinity purified polyclonal antibody (Catalog # A00306-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNAR1 at approximately 130 kDa. The expected band size for IFNAR1 is at 64 kDa.
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