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ProductsBack/IGF2R Antibody (Phospho-Ser2409) (OASG03728)

IGF2R Antibody (Phospho-Ser2409) (OASG03728)

OASG03728
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetIgf2r
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    IGF2R Antibody (Phospho-Ser2409) (OASG03728)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID16004
  • Target name
    Igf2r
  • Target description
    insulin-like growth factor 2 receptor
  • Target synonyms
    CD222, CI-MPR, M6P/IGF2R, Mpr300, cation-independent mannose-6-phosphate receptor, 300 kDa mannose 6-phosphate receptor, CI Man-6-P receptor, IGF-II receptor, IGF-II/CI-MPR, M6P/IGF2 receptor, M6PR, MPR 300, cation-independent MPR, insulin-like growth factor II receptor, mannose-6-phosphate receptor, cation independent
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Igf2r using anti-Igf2r antibody (A00951-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Igf2r antigen affinity purified polyclonal antibody (Catalog # A00951-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Igf2r at approximately 274 kDa. The expected band size for Igf2r is at 274 kDa.
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