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Western blot All lanes: IGSF11 antibody at 1.5microg/ml Lane 1: Mouse brain tissue Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 44 kDa Observed band size: 47 kDa
Western blot All lanes: IGSF11 antibody at 1.5microg/ml Lane 1: Mouse brain tissue Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 44 kDa Observed band size: 47 kDa
Western blot All lanes: IGSF11 antibody at 1.5microg/ml Lane 1: Mouse brain tissue Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 44 kDa Observed band size: 47 kDa

IGSF11 Antibody

CSB-PA691547LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetIGSF11
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Overview

  • Supplier
    Cusabio
  • Product Name
    IGSF11 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID152404
  • Target name
    IGSF11
  • Target description
    immunoglobulin superfamily member 11
  • Target synonyms
    BT-IgSF, BTIGSF, CT119, CXADRL1, Igsf13, VSIG3, immunoglobulin superfamily member 11, CXADR like 1, V-set and immunoglobulin domain containing 3, V-set and immunoglobulin domain-containing protein 3, brain and testis-specific immunoglobin superfamily protein, brain and testis-specific immunoglobulin superfamily protein, cancer/testis antigen 119
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5DX21
  • Protein Name
    Immunoglobulin superfamily member 11
  • Scientific Description
    Functions as a cell adhesion molecule through homophilic interaction. Stimulates cell growth.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IGSF11 using anti-IGSF11 antibody (A09664-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGSF11 antigen affinity purified polyclonal antibody (Catalog # A09664-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGSF11 at approximately 50 kDa. The expected band size for IGSF11 is at 46 kDa.
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