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ProductsBack/IHC-plus(tm) AES / Groucho Antibody

IHC-plus(tm) AES / Groucho Antibody

LS-B1326
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTLE5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) AES / Groucho Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    AES antibody can be used for the detection of AES by Western blot at 2 - 4 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:. ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (2 - 4 µg/ml) AES antibody can be used for the detection of AES by Western blot at 2 - 4 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID166
  • Target name
    TLE5
  • Target description
    TLE family member 5, transcriptional modulator
  • Target synonyms
    AES, AES-1, AES-2, ESP1, GRG, GRG5, Grg-5, TLE family member 5, amino-terminal enhancer of split, gp130-associated protein GAM, groucho-related protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AES using anti-AES antibody (A01757-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AES antigen affinity purified polyclonal antibody (Catalog # A01757-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AES at approximately 22KD. The expected band size for AES is at 22KD.
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