IHC-plus(tm) AIRE Antibody (aa102-115)

LifeSpan BioSciences

IHC-plus(tm) AIRE Antibody (aa102-115)

23

Immunohistochemistry: LS-B1741 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1741 was determined to be 2.5 ug/ml.. IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:32000), WB (0.5 - 1 µg/ml) Immunohistochemistry: LS-B1741 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1741 was determined to be 2.5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

AIRE

autoimmune regulator

AIRE1, APECED, APS1, APSI, PGA1, autoimmune regulator, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein

Goat

Canine, Equine, Hamster, Human, Rat

-20°C

41116161