IHC-plus(tm) AKT1S1 / PRAS40 Antibody

LifeSpan BioSciences

IHC-plus(tm) AKT1S1 / PRAS40 Antibody

23

Immunohistochemistry: LS-B2541 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2541 was determined to be 10 ug/ml. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 ug/ml. Positive control: HeLa cells; Jurkat cells.. IHC, IHC-P (10 µg/ml), IP, WB (0.1 - 1 µg/ml) Immunohistochemistry: LS-B2541 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2541 was determined to be 10 ug/ml. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 ug/ml. Positive control: HeLa cells; Jurkat cells.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.5 mg/ml

Unconjugated

AKT1S1

AKT1 substrate 1

Lobe, PRAS40, proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline rich)

Mouse

IgG1

Human

-20°C,2°C to 8°C

41116161