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ProductsBack/IHC-plus(tm) ANXA4 / Annexin IV Antibody (clone 1D3)

IHC-plus(tm) ANXA4 / Annexin IV Antibody (clone 1D3)

LS-B4279
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetANXA4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ANXA4 / Annexin IV Antibody (clone 1D3)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot (Transfected lysate) - positive control transfected 293T cell line. ELISA - Recombinant protein
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (2.5 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000) Western Blot (Transfected lysate) - positive control transfected 293T cell line. ELISA - Recombinant protein
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D3
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID307
  • Target name
    ANXA4
  • Target description
    annexin A4
  • Target synonyms
    35-beta calcimedin; annexin A4; annexin IV (placental anticoagulant protein II); annexin-4; ANX4; carbohydrate-binding protein p33/p41; chromobindin-4; endonexin I; epididymis secretory protein Li 274; HEL-S-274; lipocortin IV; P32.5; PAP-II; PIG28; placental anticoagulant protein II; PP4-X; proliferation-inducing gene 28; proliferation-inducing protein 28; protein II; ZAP36
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin-4/ANXA4 antigen affinity purified polyclonal antibody (Catalog # A04840-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin-4/ANXA4 at approximately 36 kDa. The expected band size for Annexin-4/ANXA4 is at 36 kDa.
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