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ProductsBack/IHC-plus(tm) APOL1 / Apolipoprotein L Antibody (aa261-310)

IHC-plus(tm) APOL1 / Apolipoprotein L Antibody (aa261-310)

LS-B11247
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetAPOL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) APOL1 / Apolipoprotein L Antibody (aa261-310)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 43 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:200), Peptide-ELISA (1:10000), WB (1:500 - 1:1000) Western Blot: Predicted MW 43 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8542
  • Target name
    APOL1
  • Target description
    apolipoprotein L1
  • Target synonyms
    APO-L, APOL, APOL-I, FSGS4, apolipoprotein L1, apolipoprotein L 1, apolipoprotein L1-B3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APOL1 using anti-APOL1 antibody (A01841-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human plasma tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOL1 antigen affinity purified polyclonal antibody (Catalog # A01841-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOL1 at approximately 37KD. The expected band size for APOL1 is at 44KD.
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