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ProductsBack/IHC-plus(tm) AZIN2 / Antizyme Inhibitor 2 Antibody (Internal)

IHC-plus(tm) AZIN2 / Antizyme Inhibitor 2 Antibody (Internal)

LS-B10875
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetAZIN2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) AZIN2 / Antizyme Inhibitor 2 Antibody (Internal)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suggested starting dilutions are as follows: Western blot: 1:1000-1:10000, IHC-Paraffin: 7.5 microg/ml. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.. IHC, IHC-P (7.5 µg/ml), WB (1:1000 - 1:10000) Suggested starting dilutions are as follows: Western blot: 1:1000-1:10000, IHC-Paraffin: 7.5 µg/ml. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.02 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID113451
  • Target name
    AZIN2
  • Target description
    antizyme inhibitor 2
  • Target synonyms
    ADC, AZI2, AZIB1, ODC-p, ODC1L, ODCp, antizyme inhibitor 2, ODC antizyme inhibitor-2, ODC-like protein, ODC-paralogue, arginine decarboxylase, ornithine decarboxylase like, ornithine decarboxylase-like protein, ornithine decarboxylase-paralog
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Arginine Decarboxylase/AZIN2 using anti-Arginine Decarboxylase/AZIN2 antibody (A07836). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arginine Decarboxylase/AZIN2 antigen affinity purified polyclonal antibody (Catalog # A07836) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arginine Decarboxylase/AZIN2 at approximately 45 kDa. The expected band size for Arginine Decarboxylase/AZIN2 is at 45 kDa.
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