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ProductsBack/IHC-plus(tm) BMPR2 Antibody (clone 1F12)

IHC-plus(tm) BMPR2 Antibody (clone 1F12)

LS-B9506
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetBMPR2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) BMPR2 Antibody (clone 1F12)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (1:10000), IHC, IHC-P (1:50), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1F12
  • Conjugate
    Unconjugated
  • Gene ID659
  • Target name
    BMPR2
  • Target description
    bone morphogenetic protein receptor type 2
  • Target synonyms
    BMPR-II, BMPR3, BMR2, BRK-3, POVD1, PPH1, T-ALK, bone morphogenetic protein receptor type-2, BMP type II receptor, BMP type-2 receptor, bone morphogenetic protein receptor type II, bone morphogenetic protein receptor, type II (serine/threonine kinase), type II activin receptor-like kinase, type II receptor for bone morphogenetic protein-4
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BMPR2 using anti-BMPR2 antibody (A00324-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMPR2 antigen affinity purified polyclonal antibody (Catalog # A00324-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMPR2 at approximately 130KD. The expected band size for BMPR2 is at 115KD.
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