IHC-plus(tm) CCL20 / MIP-3-Alpha Antibody

LifeSpan BioSciences

IHC-plus(tm) CCL20 / MIP-3-Alpha Antibody

23

Immunohistochemistry: LS-B7409 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3alpha when used with biotinylated antigen affinity purified anti-Human MIP-3alpha (LS-C104612) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 0.25-0.50 ng/lane, under non-reducing conditions.. ELISA (2 - 4 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.2 - 0.4 µg/ml) Immunohistochemistry: LS-B7409 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3alpha when used with biotinylated antigen affinity purified anti-Human MIP-3alpha (LS-C104612) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 0.25-0.50 ng/lane, under non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Unconjugated

CCL20

C-C motif chemokine ligand 20

CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38, C-C motif chemokine 20, CC chemokine LARC, beta chemokine exodus-1, chemokine (C-C motif) ligand 20, liver and activation-regulated chemokine, macrophage inflammatory protein 3 alpha, small inducible cytokine subfamily A (Cys-Cys), member 20, small-inducible cytokine A20

Mouse

IgG1

Human

-20°C,2°C to 8°C,RT

41116161