IHC-plus(tm) CD14 Antibody (aa71-84)

LifeSpan BioSciences

IHC-plus(tm) CD14 Antibody (aa71-84)

23

Immunohistochemistry: LS-B3012 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3012 was determined to be 4 ug/ml.. IHC, IHC-P (4 µg/ml), Peptide-ELISA (1:128000), WB (1 - 3 µg/ml) Immunohistochemistry: LS-B3012 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3012 was determined to be 4 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

CD14

CD14 molecule

monocyte differentiation antigen CD14, my23 antigen, myeloid cell-specific leucine-rich glycoprotein

Goat

Human

-20°C

41116161