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IHC-plus(tm) CIITA Antibody

LS-B5490
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCIITA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CIITA Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    CIITA antibody can be used for detection of CIITA by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 124 kDa Observed: 125 kDa. ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) CIITA antibody can be used for detection of CIITA by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 124 kDa Observed: 125 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4261
  • Target name
    CIITA
  • Target description
    class II major histocompatibility complex transactivator
  • Target synonyms
    C2TA, CIITAIV, MHC2D1, MHC2TA, NLRA, MHC class II transactivator, MHC class II transactivator type I, MHC class II transactivator type III, NLR family, acid domain containing, nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CIITA using anti-CIITA antibody (A01556-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIITA antigen affinity purified polyclonal antibody (Catalog # A01556-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIITA at approximately 123 kDa. The expected band size for CIITA is at 123 kDa.
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