IHC-plus(tm) CLCF1 Antibody

LifeSpan BioSciences

IHC-plus(tm) CLCF1 Antibody

23

Immunohistochemistry: LS-B8220 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NNT-1/BCSF-3 (LS-C104491) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNNT-1/BCSF-3. Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B8220 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NNT-1/BCSF-3 (LS-C104491) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNNT-1/BCSF-3. Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

...

CLCF1

cardiotrophin like cytokine factor 1

BSF-3, BSF3, CISS2, CLC, NNT-1, NNT1, NR6, cardiotrophin-like cytokine factor 1, B-cell stimulatory factor 3, B-cell-stimulating factor 3, CRLF1 associated cytokine-like factor 1, neurotrophin-1/B-cell stimulating factor-3, novel neurotrophin-1

Rabbit

Human

-20°C,2°C to 8°C,RT

12352203