IHC-plus(tm) CLU / Clusterin Antibody

LifeSpan BioSciences

IHC-plus(tm) CLU / Clusterin Antibody

23

Immunohistochemistry: LS-B2218 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2218 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Detects human clusterin a chain (40kD) in RIPA lysates from HeLa cells, human serum and MCF-7 cell lysates.. IHC, IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml) Immunohistochemistry: LS-B2218 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2218 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Detects human clusterin a chain (40kD) in RIPA lysates from HeLa cells, human serum and MCF-7 cell lysates.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.5 mg/ml

Unconjugated

CLU

clusterin

AAG4, APO-J, APOJ, CLI, CLU1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, clusterin, aging-associated protein 4, apolipoprotein J, complement cytolysis inhibitor, complement lysis inhibitor, complement-associated protein SP-40,40, epididymis secretory sperm binding protein, ku70-binding protein 1, sulfated glycoprotein 2, testosterone-repressed prostate message 2

Mouse

IgG1

Human

-20°C,2°C to 8°C

41116161