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ProductsBack/IHC-plus(tm) COL6A2 / Collagen VI Alpha 2 Antibody (clone 2C5-F2)

IHC-plus(tm) COL6A2 / Collagen VI Alpha 2 Antibody (clone 2C5-F2)

LS-B11119
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCOL6A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) COL6A2 / Collagen VI Alpha 2 Antibody (clone 2C5-F2)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein. Immunohistochemistry: Paraffin.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (10 µg/ml), WB Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein. Immunohistochemistry: Paraffin.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2C5-F2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1292
  • Target name
    COL6A2
  • Target description
    collagen type VI alpha 2 chain
  • Target synonyms
    BTHLM1, BTHLM1B, PP3610, UCMD1, UCMD1B, collagen alpha-2(VI) chain, collagen VI, alpha-2 polypeptide, collagen, type VI, alpha 2, epididymis secretory sperm binding protein, human mRNA for collagen VI alpha-2 C-terminal globular domain
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen VI/COL6A2 antigen affinity purified polyclonal antibody (Catalog # A03194-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen VI/COL6A2 at approximately 150 kDa. The expected band size for Collagen VI/COL6A2 is at 109 kDa.
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