IHC-plus(tm) Collagen I Antibody

LifeSpan BioSciences

IHC-plus(tm) Collagen I Antibody

23

Immunohistochemistry: LS-B3653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40.. ELISA, IF, IHC, IHC-P (1:100) Immunohistochemistry: LS-B3653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40. WB

ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

...

Rabbit

IgG

Human

2°C to 8°C or -20°C

12352203