IHC-plus(tm) Collagen VI Antibody (Biotin)

LifeSpan BioSciences

IHC-plus(tm) Collagen VI Antibody (Biotin)

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Recent testing with this antibody has shown that it has some cross reactivity with COL6A2. Immunohistochemistry: LS-B380 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B380 was determined to be 1.25 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ELISA (1:10000 - 1:50000), IHC, IHC-P (1.25 µg/ml), IP (1:100), WB (1:5000 - 1:10000) Recent testing with this antibody has shown that it has some cross reactivity with COL6A2. Immunohistochemistry: LS-B380 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B380 was determined to be 1.25 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Biotin

Rabbit

IgG

Bovine, Human, Mammals

-20°C,2°C to 8°C

41116161