IHC-plus(tm) Collagen VI Antibody
LS-B696
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mammals
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) Collagen VI Antibody
- Delivery Days Customer23
- Application Supplier NotePercentage of reactivty with all isoforms of COL6 has not been determined. Immunohistochemistry: LS-B696 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B696 was determined to be 2.5 ug/ml.. ELISA (1:5000 - 1:20000), IHC, IHC-P (2.5 µg/ml), IP (1:100), WB (1:500 - 1:5000) Percentage of reactivty with all isoforms of COL6 has not been determined. Immunohistochemistry: LS-B696 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B696 was determined to be 2.5 ug/ml.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityBovine, Human, Mammals
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC41116161