IHC-plus(tm) Complement C2 Antibody

LifeSpan BioSciences

IHC-plus(tm) Complement C2 Antibody

23

Immunohistochemistry: LS-B2639 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2639 was determined to be 10 ug/ml. Western Blot: After SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions.. ELISA (1:2000), IHC, IHC-P (10 µg/ml), WB (1:125) Immunohistochemistry: LS-B2639 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2639 was determined to be 10 ug/ml. Western Blot: After SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

1 mg/ml

Unconjugated

C2

complement C2

ARMD14, CO2, complement C2, C3/C5 convertase, complement component 2, complement component C2

Mouse

IgG1

Human

2°C to 8°C or -20°C

41116161